Mouse Undercarboxylated Osteocalcin (ucOC) ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠羧化不全骨鈣素(ucOC)酶聯免疫試劑盒（CSB-E17681m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的ucOC含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，為骨生物學研究提供可靠工具，特別適用于骨質疏松動物模型構建、骨代謝調控機制研究、藥物干預效果評價等科研領域，其優化的檢測體系能有效支持血清等復雜樣本中微量ucOC的穩定檢出，為探索骨骼系統疾病發生發展機制提供數據支撐。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

ucOC
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ucOC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	80-90
1:10	Average %	92
	Range %	86-96
1:20	Average %	94
	Range %	87-100
1:40	Average %	97
	Range %	92-101

回收率：

The recovery of mouse ucOC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	90	87-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.