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Your Good Partner in Biology Research

Mouse Substance P,SP ELISA Kit

  • 中文名稱:
    小鼠P物質(SP)酶聯免疫試劑盒
  • 貨號:
    CSB-E08359m
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    小鼠P物質(SP)酶聯免疫試劑盒(CSB-E08359m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的SP含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL,廣泛用于基礎科研領域,如探究神經系統疾病模型中SP的分泌動態、評估藥物對神經肽表達的調控作用,或解析炎癥反應與神經信號通路的關聯機制,為神經生物學、免疫學及藥理學研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    SP
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    3.12 pg/mL-200 pg/mL
  • 靈敏度:
    0.78 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %96
    Range %92-104
    1:200Average %94
    Range %89-98
    1:400Average %89
    Range %86-96
    1:800Average %94
    Range %89-97
  • 回收率:
    The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8682-94
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.615 2.514 2.565 2.381
    1001.887 1.786 1.837 1.653
    501.093 1.073 1.083 0.899
    250.668 0.657 0.663 0.479
    12.50.436 0.447 0.442 0.258
    6.250.311 0.325 0.318 0.134
    3.120.241 0.246 0.244 0.060
    00.180 0.187 0.184  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 2 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:其他 1:200

產品評價: 我用CSB-E08359m檢測正常血清,其od值為0.7/0.604/0.559/0.639/0.577/0.671

By 張老師

樣品類型:血清

樣品信息:小鼠

稀釋比:其他 1:200

產品評價: 我用CSB-E08359m檢測正常血清,標準曲線的OD值為 2.8995 2.483 1.703 0.9295 0.532 0.342 0.177 0.0975 ,樣本的OD值(樣本 OD可以提供大概區間)為0.6~1.0,產品好。

By 張老師