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  4. Mouse Serine protease HTRA1(HTRA1) ELISA kit

Mouse Serine protease HTRA1(HTRA1) ELISA kit

  • 中文名稱：
    小鼠絲氨酸蛋白酶HTRA1(HTRA1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL010901MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠絲氨酸蛋白酶HTRA1(HTRA1)酶聯免疫試劑盒（CSB-EL010901MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HTRA1含量。HTRA1是一種絲氨酸蛋白酶。其與多種疾病相關，如年齡相關性黃斑變性等。研究機制上，它可通過調控細胞外基質、生長因子等發揮作用，參與細胞的增殖、凋亡等過程，針對它的研究有助于探索疾病的發病機制和潛在治療靶點。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，支持科研人員便捷地開展動物模型中的HTRA1動態監測，如中樞神經系統損傷修復研究、腫瘤微環境分析或心血管疾病機制探索，為蛋白表達調控、藥物干預效果評估等實驗提供可靠數據支持，適用于分子生物學、基礎醫學等領域的非診斷性研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Htra1 ELISA Kit; Htra ELISA Kit; Prss11 ELISA Kit; Serine protease HTRA1 ELISA Kit; EC 3.4.21.- ELISA Kit; High-temperature requirement A serine peptidase 1 ELISA Kit; Serine protease 11 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HTRA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 85-93
    1:2 Average % 95
    Range % 90-100
    1:4 Average % 91
    Range % 86-95
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of mouse HTRA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 100-110
    EDTA plasma (n=4) 93 89-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    5000 2.635 2.492 2.564 2.374
    2500 2.001 1.903 1.952 1.762
    1250 1.329 1.357 1.343 1.153
    625 0.814 0.860 0.837 0.647
    312 0.547 0.519 0.533 0.343
    156 0.352 0.338 0.345 0.155
    78 0.263 0.271 0.267 0.077
    0 0.186 0.194 0.190 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
  • 基因功能參考文獻：
    1. transfection of cells with a constitutively active rapamycin-resistant p70S6K mutant could restore the mineralizing capacity of HtrA1-deficient mouse adipose-derived stromal cells. PMID: 26950191
    2. HtrA1 plays important roles in the differentiation of trophoblasts from Tpbpa-positive precursors in the ectoplacental cone PMID: 25446274
    3. The HtrA1 overexpression and mainstream cigarette smoke can independently lead to CNV. The HtrA1 gene is a strong risk factor for wet AMD PMID: 25205867
    4. the inhibitory effect of IFN-gamma on HTRA1 expression was evidenced in collagen-induced arthritis (CIA) mouse models and in human RA synovial cells. PMID: 24907345
    5. Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation. PMID: 24040176
    6. This study offers new insights into the regulation of HTRA-1 expression via LPS/TLR-4 and the role of HTRA-1 in rheumatoid arthrisis pathogenesis. PMID: 23982886
    7. suggest a critical role for LRP1 in maintaining the integrity of vessels by regulating protease activity as well as matrix deposition by modulating HtrA1 and connective tissue growth factor protein levels. PMID: 23868935
    8. a critical role of HTRA1 in the regulation of angiogenesis via TGF-beta signaling and identified GDF6 as a novel disease gene for AMD. PMID: 22049084
    9. showed that increased HTRA1 induced cardinal features of polypoidal choroidal vasculopathy, including branching networks of choroidal vessels, polypoidal lesions, severe degeneration of the elastic laminae, and tunica media of choroidal vessels PMID: 21844367
    10. HtrA1 may disrupt the pericellular matrix network, resulting in alteration of chondrocyte metabolisms. This eventually leads to osteoarthritis. PMID: 20238298
    11. HtrA1 has a role in promoting anoikis by attenuating activation of EGFR/AKT pathway that may contribute to its metastasis suppression capacity PMID: 20388781
    12. During embryo development, HtrA1 was expressed in specific areas where signaling by Tgfbeta family proteins plays important regulatory roles PMID: 14973287
    13. HtrA1 as a protease potentially important for trophoblast differentiation/invasion and uterine decidual regression during placental development PMID: 15861393
    14. bone matrix shows a high level of HtrA1 protein deposition akin to that of TGF-beta, suggesting a close functional interaction between TGF-beta and HtrA1 PMID: 15993670
    15. highly upregulated during mucosal mast cell differentiation PMID: 17292962
    16. HtrA1 and PRSS35 may be involved in ovulation and corpus luteum formation and regression, and that PRSS23 may play a role in follicular atresia PMID: 18566130

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  • 亞細胞定位：
    Cell membrane. Secreted. Cytoplasm, cytosol.
  • 蛋白家族：
    Peptidase S1C family
  • 組織特異性：
    In the brain, mainly expressed in cortical areas both in glial cells and neurons (at protein level). In bones, deposited in the matrix, with higher level in newly formed bone compared to fully calcified bone (at protein level). Also expressed in the tendo
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