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  4. Mouse Platelet-derived growth factor subunit B(PDGFB) ELISA kit

Mouse Platelet-derived growth factor subunit B(PDGFB) ELISA kit

  • 中文名稱：
    小鼠血小板衍生生長因子β(PDGFB)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL017709MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠血小板衍生生長因子β(PDGFB)酶聯(lián)免疫試劑盒（CSB-EL017709MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PDGFB含量。PDGFB 即血小板衍生生長因子 B，在細胞生長、增殖和分化等過程中扮演關(guān)鍵角色。其異常表達與腫瘤生長、纖維化疾病等密切相關(guān)。研究機制主要聚焦于其與受體結(jié)合后激活下游信號通路，調(diào)控細胞行為，為相關(guān)疾病治療提供靶點。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，其動態(tài)監(jiān)測可為心血管疾病、纖維化疾病及腫瘤機制研究提供重要數(shù)據(jù)支持；適用于體外實驗中PDGFB的表達水平分析，例如細胞因子調(diào)控研究、疾病模型病理分析或藥物干預(yù)效果評估等科研場景；可為細胞實驗、動物模型及離體組織研究提供穩(wěn)定可靠的定量檢測工具，助力科研人員探索PDGFB在生物學(xué)過程中的分子機制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    93.75 pg/mL-6000 pg/mL
  • 靈敏度：
    23.44 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGFB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    86

    Range %

    82-91

    1:2

    Average %

    88

    Range %

    83-93

    1:4

    Average %

    92

    Range %

    85-99

    1:8

    Average %

    85

    Range %

    81-90

  • 回收率：

    The recovery of mouse PDGFB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    85

    81-90

    EDTA plasma (n=4)

    90

    83-97

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    6000

    2.090

    2.034

    2.062

    1.901

    3000

    1.308

    1.227

    1.267

    1.106

    1500

    0.864

    0.828

    0.846

    0.685

    750

    0.526

    0.519

    0.523

    0.361

    375

    0.334

    0.321

    0.327

    0.166

    187.5

    0.243

    0.253

    0.248

    0.087

    93.75

    0.201

    0.194

    0.198

    0.036

    0

    0.158

    0.165

    0.161

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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