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  4. Mouse N-myc-interactor(NMI) ELISA kit

Mouse N-myc-interactor(NMI) ELISA kit

  • 中文名稱：
    小鼠N-Myc互作蛋白(NMI)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL015893MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠N-Myc互作蛋白(NMI)酶聯(lián)免疫試劑盒（CSB-EL015893MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的NMI含量。小鼠N-Myc互作蛋白(NMI)是一種與NMYC和CMYC相互作用的蛋白質(zhì)，參與細胞周期、凋亡和轉(zhuǎn)化。NMI通過增強STAT介導(dǎo)的轉(zhuǎn)錄，在細胞因子IL2和IFN-γ的刺激下發(fā)揮作用，并在多種癌癥中表達上調(diào)，可能成為癌癥治療的新靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品適用于腫瘤生物學(xué)、免疫調(diào)控等基礎(chǔ)研究領(lǐng)域，可應(yīng)用于小鼠模型中的腫瘤微環(huán)境分析、免疫細胞功能研究或藥物干預(yù)后NMI表達動態(tài)監(jiān)測，為探索NMI在腫瘤抑制、炎癥反應(yīng)及細胞信號轉(zhuǎn)導(dǎo)中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    NmiN-myc-interactor ELISA Kit; Nmi ELISA Kit; N-myc and STAT interactor ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NMI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 88-102
    1:2 Average % 92
    Range % 88-97
    1:4 Average % 94
    Range % 85-99
    1:8 Average % 100
    Range % 91-105
  • 回收率：
    The recovery of mouse NMI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-98
    EDTA plasma (n=4) 99 91-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.611 2.569 2.590 2.470
    500 2.392 2.314 2.353 2.233
    250 1.979 1.898 1.939 1.819
    125 1.130 1.105 1.118 0.998
    62.5 0.645 0.622 0.634 0.514
    31.2 0.425 0.434 0.430 0.310
    15.6 0.315 0.307 0.311 0.191
    0 0.122 0.118 0.120 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts as a signaling pathway regulator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis. Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription. In response to interferon IFN-alpha, associates in a complex with transcriptional regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35. In complex with IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Negatively regulates virus-triggered type I interferon/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering with cellular antiviral responses. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellular space during cell injury or pathogen invasion. Macrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines.
  • 基因功能參考文獻：
    1. Damage-associated molecular patterns (DAMP) are important mediators of innate immunity. Here the authors show that N-myc and STAT interactor (NMI) and interferon-induced protein 35 (IFP35) act as DAMPs to promote inflammation by activating macrophages via the Toll-like receptor 4 and NF-kappaB pathways. PMID: 29038465
  • 亞細胞定位：
    Cytoplasm. Nucleus. Secreted.
  • 蛋白家族：
    NMI family
  • 組織特異性：
    Expressed in macrophages.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:64685

    STRING: 10090.ENSMUSP00000028314

    UniGene: Mm.7491