Mouse Chordin-like protein 1(CHRDL1) ELISA kit

Mouse Chordin-like protein 1(CHRDL1) ELISA kit

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中文名稱：

小鼠腱蛋白樣蛋白1(CHRDL1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL005378MO
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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產(chǎn)品描述：

小鼠腱蛋白樣蛋白1(CHRDL1)酶聯(lián)免疫試劑盒（CSB-EL005378MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CHRDL1含量。小鼠腱蛋白樣蛋白1（CHRDL1）是一種重要的分泌性發(fā)育介導(dǎo)蛋白，在多種組織中表達(dá)，具有調(diào)節(jié)細(xì)胞命運(yùn)和促進(jìn)神經(jīng)元分化的功能。其研究機(jī)制涉及與BMP4結(jié)合作為拮抗劑，阻斷受體相互作用，并可能參與多種生理和病理過程，如神經(jīng)發(fā)育和癌癥預(yù)后。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，本試劑盒適用于體外研究CHRDL1在神經(jīng)生物學(xué)、骨骼發(fā)育或腫瘤模型中的表達(dá)水平變化。為探索CHRDL1的分子功能及調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Chrdl1 ELISA kit; Ng1 ELISA kit; Nrln1Chordin-like protein 1 ELISA kit; Neuralin-1 ELISA kit; Neurogenesin-1 ELISA kit; Ventroptin ELISA kit
縮寫：

CHRDL1
Uniprot No.：

Q920C1
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	101
	Range %	97-106
1:4	Average %	98
	Range %	92-103
1:8	Average %	86
	Range %	82-89

回收率：

The recovery of mouse CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	89	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.377	2.356	2.367	2.217
500	1.599	1.687	1.643	1.493
250	0.933	0.997	0.965	0.815
125	0.541	0.568	0.555	0.405
62.5	0.355	0.326	0.341	0.191
31.2	0.222	0.236	0.229	0.079
7.8	0.154	0.167	0.161	0.011
0	0.144	0.156	0.150

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development.
基因功能參考文獻(xiàn)：
1. CHRDL-1 is a new regulatory target of miR-134 during cortical neuronal differentiation; this is a novel role for miR-134 in the modulation of cortical development. PMID: 21228099
2. Reperfusion injury of proximal kidney tubules causes a reduction of Chrdl1 expression. PMID: 19357253
亞細(xì)胞定位：

Secreted.
組織特異性：

Expressed in heart, brain, lung, liver, kidney and testis.
數(shù)據(jù)庫鏈接：

KEGG: mmu:83453

STRING: 10090.ENSMUSP00000056193

UniGene: Mm.157697

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.