Human β-hexosaminidase A,β-Hex A ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人β氨基己糖苷酶A(β-Hex A)酶聯免疫試劑盒（CSB-E09462h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的βHex A含量。βHex A是一種重要靶點。它在某些疾病發病機制中起關鍵作用，如泰-薩克斯病等。研究機制方面，主要聚焦于其在細胞內代謝產物的清除過程，通過解析其結構與功能，試圖尋找調節它的方法，以研發治療相關疾病的藥物。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，該產品適用于科研領域中對β - Hex A表達水平的定量分析，可用于細胞模型、動物實驗或人體生物樣本中酶活性變化的研究，為探索溶酶體貯積癥發病機制、代謝通路調控或潛在治療策略的體外評估提供高效工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

beta-hexosaminidase A, beta-Hex A
縮寫：

βHex A
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

1.56 mIU/mL-100 mIU/mL
靈敏度：

0.39 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
Range %	85-94
1:2	Average %	93
Range %	88-94
1:4	Average %	105
Range %	100-112
1:8	Average %	86
Range %	80-92

回收率：

The recovery of human β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.