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  4. Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1,Tie-1 ELISA kit

Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1,Tie-1 ELISA kit

  • 中文名稱：
    人含免疫球蛋白樣環(huán)和上皮生長因子樣域酪氨酸激酶1(Tie-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04728h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人含免疫球蛋白樣環(huán)和上皮生長因子樣域酪氨酸激酶1(Tie-1)酶聯(lián)免疫試劑盒（CSB-E04728h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TIE1含量。TIE1是一個靶點。它在血管生成等生理過程中發(fā)揮作用。研究發(fā)現(xiàn)其與血管內(nèi)皮細胞的存活、增殖等機制密切相關。深入研究TIE1有助于了解血管相關疾病發(fā)病機制，為開發(fā)針對該靶點治療血管疾病的藥物提供方向。試劑盒檢測范圍為0.94 ng/mL-60 ng/mL，適用于基礎科研中探索血管生成機制、評估心血管疾病模型血管內(nèi)皮功能變化，或分析腫瘤微環(huán)境中的血管重塑等相關研究，為血管生物學、腫瘤學及病理生理學領域的體外研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    JKT 14 ELISA Kit; JTK14 ELISA Kit; TIE ELISA Kit; Tie1 ELISA Kit; TIE1_HUMAN ELISA Kit; Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 ELISA Kit; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains ELISA Kit; Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 ELISA Kit; Tyrosine protein kinase receptor Tie 1 ELISA Kit; Tyrosine-protein kinase receptor Tie-1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.94 ng/mL-60 ng/mL
  • 靈敏度：
    0.23 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Tie-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 87  
    Range % 83-92  
    1:10 Average % 106  
    Range % 101-110  
    1:20 Average % 99  
    Range % 95-103  
    1:40 Average % 98  
    Range % 94-103  
  • 回收率：
    The recovery of human Tie-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 93-99  
    EDTA plasma (n=4) 91 87-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    60 2.531 2.501 2.516 2.402  
    30 2.018 2.121 2.070 1.956  
    15 1.505 1.568 1.537 1.423  
    7.5 1.040 1.068 1.054 0.940  
    3.75 0.657 0.687 0.672 0.558  
    1.88 0.421 0.432 0.427 0.313  
    0.94 0.271 0.290 0.281 0.167  
    0 0.112 0.115 0.114    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
  • 基因功能參考文獻：
    1. We identified colorectal cancer as a novel Tie1-expressing tumor, with Tie1-positive cells hardly detectable in the normal intestine. Tie1 expression did not influence cancer cell proliferation in regular in vitro cultures, but significantly affected malignant growth of transplanted tumors in vivo. PMID: 28464467
    2. T794A-expressing human umbilical vein endothelial cells formed significantly shorter tubes with fewer branches in three-dimensional Matrigel cultures, but did not alter Tie1 or Tie2 tyrosine phosphorylation or downstream signaling. PMID: 26436659
    3. The decreasing expression of Tie1 may play an important role in the pathogenesis of primary lower extremity varicose veins. PMID: 22987233
    4. Data propose that EndMT associated with Tie1 downregulation participates in the pathological development of stroma observed in tumours. PMID: 22421998
    5. The Tie-1 immunoreactivity was dominantly observed in the heamangiogenic cells and cells cords, whereas the matured villi showed immunoreactivity only in other components. PMID: 20164029
    6. A natural antisense transcript was identified for tyrosine kinase containing immunoglobulin and epidermal growth factor homology domain-1 (tie-1), tie-1AS long noncoding RNA in zebrafish, mouse, and humans. PMID: 19880500
    7. high expression independently associated with shorter survival in patients with early chronic phase CML PMID: 11920509
    8. Overexpression and activation of Tie1 is associated with breast and colonic neopalsms PMID: 17786322
    9. the trophoblastic shell of the very early human placenta, as well as endothelial cells and ACC exhibited strong staining intensity for Tie-1 PMID: 18675456
    10. Tie-1 has an inflammatory function in endothelial cells. PMID: 19236867

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  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Tie subfamily
  • 組織特異性：
    Specifically expressed in developing vascular endothelial cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11809

    OMIM: 600222

    KEGG: hsa:7075

    STRING: 9606.ENSP00000361554

    UniGene: Hs.78824