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  4. Human serum amyloid A2 (SAA2) ELISA kit

Human serum amyloid A2 (SAA2) ELISA kit

  • 中文名稱：
    人血清淀粉樣蛋白A2(SAA2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL020657HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血清淀粉樣蛋白A2(SAA2)酶聯(lián)免疫試劑盒（CSB-EL020657HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SAA2含量。SAA2即血清淀粉樣蛋白A2，屬于急性時相蛋白。在炎癥、感染等刺激下其表達水平會急劇上升。研究機制方面，它參與免疫調(diào)節(jié)，可趨化免疫細胞。還與代謝紊亂、心血管疾病等發(fā)病機制有一定聯(lián)系，是潛在的疾病診斷與治療靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，為探索炎癥相關(guān)疾病機制（如動脈粥樣硬化、類風濕性關(guān)節(jié)炎等）提供可靠工具，亦可用于藥物干預(yù)或基因編輯模型中SAA2表達變化的科研評估，滿足體外實驗、動物模型及細胞研究等多場景需求。適用于血清、血漿等體液樣本中SAA2的動態(tài)監(jiān)測，或組織勻漿中蛋白表達的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    SAA2
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human SAA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:50

    Average %

    93

    Range %

    88-99

    1:100

    Average %

    89

    Range %

    82-96

    1:200

    Average %

    99

    Range %

    94-105

    1:400

    Average %

    92

    Range %

    86-98

  • 回收率：

    The recovery of human SAA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    91-104

    EDTA plasma (n=4)

    91

    84-97

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.175

    2.234

    2.205

    2.066

    10

    1.784

    1.667

    1.726

    1.587

    5

    1.303

    1.308

    1.306

    1.167

    2.5

    0.829

    0.869

    0.849

    0.710

    1.25

    0.577

    0.601

    0.589

    0.450

    0.625

    0.432

    0.453

    0.443

    0.304

    0.312

    0.267

    0.280

    0.274

    0.135

    0

    0.143

    0.135

    0.139

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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