Human pro-prostate specific antigen (Pro-PSA) ELISA kit

產品詳情

<ul id="igggk"></ul>

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人前列腺特異性抗原前體(Pro-PSA)酶聯免疫試劑盒（CSB-ET027786HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Pro-PSA含量。Pro-PSA即前列腺特異性抗原前體，在前列腺癌的診斷和病情評估中有重要意義。其研究機制主要是通過檢測體內Pro-PSA含量，與總PSA等指標結合，能更準確地鑒別前列腺癌與良性前列腺疾病，提高早期診斷的準確性。試劑盒檢測范圍為125 pg/mL-4000 pg/mL，該產品適用于探索前列腺疾病分子機制、評估生物標志物表達特征、篩選潛在治療靶點等基礎研究，也可用于細胞模型或動物模型中Pro - PSA的動態監測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Pro-PSA
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 pg/mL-4000 pg/mL
靈敏度：

62.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	90
	Range %	84-94
1:4	Average %	105
	Range %	98-108
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human Pro-PSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-94
EDTA plasma (n=4)	93	89-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Serum proPSA as a marker for reducing repeated prostate biopsy numbers Sema Nur Ayy謀ld謀z.et al,Turkish Journal of Biochemistry T眉rk Biyokimya Dergisi,2017