Human oxidized low density lipoprotein antibody,OLAb ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人氧化低密度脂蛋白抗體(OLAb)酶聯(lián)免疫試劑盒（CSB-E08797h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ox-LDL Ab含量。ox-LDL Ab即氧化低密度脂蛋白抗體。其背景在于氧化低密度脂蛋白與動(dòng)脈粥樣硬化等疾病密切相關(guān)，機(jī)體產(chǎn)生相應(yīng)抗體。研究機(jī)制上，主要探索其與疾病進(jìn)程的關(guān)聯(lián)，如在斑塊形成、炎癥反應(yīng)中的作用，以明確其作為診斷標(biāo)志物或治療靶點(diǎn)的價(jià)值。試劑盒檢測范圍為47 μU/mL-3000 μU/mL，該產(chǎn)品適用于心血管疾病模型構(gòu)建、代謝異常相關(guān)研究或炎癥反應(yīng)機(jī)制分析等科研場景，為評估氧化脂蛋白在疾病發(fā)展中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ox-LDL Ab
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

47 μU/mL-3000 μU/mL
靈敏度：

11.75 μU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OLAb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	87-98
1:2000	Average %	93
	Range %	91-99
1:4000	Average %	95
	Range %	90-102
1:8000	Average %	92
	Range %	86-98

回收率：

The recovery of human OLAb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-98
EDTA plasma (n=4)	96	91-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.