Human beta-crosslaps (bCTx) ELISA kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人β膠原交聯(lián)(bCTx)酶聯(lián)免疫試劑盒（CSB-EQ027521HU）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的bCTx含量。試劑盒檢測(cè)范圍為125 pg/mL-2000 pg/mL，在科研場(chǎng)景中，本試劑盒可廣泛應(yīng)用于骨代謝機(jī)制研究、抗骨質(zhì)疏松藥物開發(fā)評(píng)估、骨骼疾病動(dòng)物模型評(píng)價(jià)等領(lǐng)域，為骨轉(zhuǎn)換相關(guān)的基礎(chǔ)研究提供關(guān)鍵數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

bCTx
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測(cè)范圍：

125 pg/mL-2000 pg/mL
靈敏度：

125 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human bCTx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	96-108
1:2	Average %	88
	Range %	83-92
1:4	Average %	101
	Range %	93-104
1:8	Average %	106
	Range %	100-110

回收率：

The recovery of human bCTx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	82-97
EDTA plasma (n=4)	93	87-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.