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  4. Human Transgelin-2(TAGLN2) ELISA kit

Human Transgelin-2(TAGLN2) ELISA kit

  • 中文名稱：
    人轉(zhuǎn)膠蛋白2(TAGLN2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL023106HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人轉(zhuǎn)膠蛋白2(TAGLN2)酶聯(lián)免疫試劑盒（CSB-EL023106HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、細(xì)胞裂解物樣本中的TAGLN2含量。TAGLN2是一種與肌動蛋白結(jié)合的蛋白，被認(rèn)為具有腫瘤抑制功能。其研究機(jī)制涉及調(diào)節(jié)T細(xì)胞脂質(zhì)代謝，通過促進(jìn)脂肪酸結(jié)合蛋白5（FABP5）的運(yùn)輸至T細(xì)胞表面，增強(qiáng)T細(xì)胞在腫瘤微環(huán)境中的抗腫瘤功能。TAGLN2的表達(dá)受腫瘤微環(huán)境中的內(nèi)質(zhì)網(wǎng)應(yīng)激反應(yīng)調(diào)節(jié)，其過表達(dá)可能有助于提高癌癥免疫療法的療效。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，科研人員可利用開展疾病相關(guān)分子機(jī)制研究，例如通過檢測不同處理條件下細(xì)胞裂解液或體液樣本中TAGLN2的動態(tài)變化，探索其在細(xì)胞運(yùn)動、炎癥反應(yīng)或代謝調(diào)控中的功能，也可用于藥物開發(fā)過程中靶點(diǎn)蛋白表達(dá)水平的定量評估。本產(chǎn)品適用于生命科學(xué)基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)領(lǐng)域的蛋白定量需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CDABP0035 ELISA Kit; epididymis tissue protein Li 7e ELISA Kit; HA1756 ELISA Kit; KIAA0120 ELISA Kit; SM22-alpha homolog ELISA Kit; TAGL2_HUMAN ELISA Kit; Tagln2 ELISA Kit; Transgelin-2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell lysates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-92
    1:2Average %101
    Range %98-103
    1:4Average %92
    Range %85-96
    1:8Average %99
    Range %93-102
  • 回收率：
    The recovery of human TAGLN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9592-98
    EDTA plasma (n=4)9692-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    202.398 2.367 2.383 2.228
    101.672 1.576 1.624 1.469
    51.071 1.062 1.067 0.912
    2.50.624 0.618 0.621 0.466
    1.250.427 0.411 0.419 0.264
    0.6250.319 0.306 0.313 0.158
    0.3120.251 0.240 0.246 0.091
    00.156 0.154 0.155 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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