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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human Mucin 2(MUC2) ELISA Kit
  • 中文名稱(chēng)：
    人粘蛋白2(MUC2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13122h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人粘蛋白2(MUC2)酶聯(lián)免疫試劑盒（CSB-E13122h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的MUC2含量。MUC2是一種黏蛋白，在人體胃腸道等黏膜表面廣泛存在，形成黏液層，保護(hù)上皮細(xì)胞。研究顯示其表達(dá)異常與腸道炎癥、腫瘤等疾病密切相關(guān)。機(jī)制上，它可通過(guò)調(diào)節(jié)黏液屏障功能、影響細(xì)胞信號(hào)傳導(dǎo)等參與疾病發(fā)生發(fā)展過(guò)程，是潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于科研領(lǐng)域探究胃腸道疾病機(jī)制、評(píng)估細(xì)胞或組織模型中粘液層功能變化、篩選調(diào)控MUC2表達(dá)的化合物等研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Intestinal mucin 2 ELISA Kit; Intestinal mucin-2 ELISA Kit; MLP ELISA Kit; MUC 2 ELISA Kit; MUC-2 ELISA Kit; Muc2 ELISA Kit; MUC2_HUMAN ELISA Kit; Mucin 2 ELISA Kit; Mucin 2 intestinal ELISA Kit; Mucin 2 intestinal/tracheal ELISA Kit; Mucin 2 oligomeric mucus/gel forming ELISA Kit; Mucin 2 precursor ELISA Kit; Mucin 2 tracheal ELISA Kit; Mucin like protein ELISA Kit; Mucin-2 ELISA Kit; Mucin2 ELISA Kit; SMUC ELISA Kit
  • 縮寫(xiě)：
    MUC2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-96
    1:2Average %101
    Range %99-105
    1:4Average %94
    Range %90-98
    1:8Average %89
    Range %85-94
  • 回收率：
    The recovery of human MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8480-88
    EDTA plasma (n=4)9590-99
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.531 2.481 2.506 2.364
    1002.032 1.961 1.997 1.855
    501.448 1.401 1.425 1.283
    250.876 0.823 0.850 0.708
    12.50.597 0.623 0.610 0.468
    6.250.391 0.402 0.397 0.255
    3.120.233 0.217 0.225 0.083
    00.144 0.139 0.142 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MUC2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled MUC2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Please provide then following information regarding Human Mucin 2, MUC2 ELISA Kit (CSB-E13122h)
1. Are the antibodies in the kit monoclonal or polyclonal ?
2. If the antibodies are polyclonal, please kindly let us know the HOST, antigen, and the purification method
(Affinity chromatography or protein A (G) etc.)
3. what is stop solution ? 1M Hydrochloric Acid ?
4. If the kit includes vaculovirus or other virus expressions,
please kindly let us know which items in the kit include it.
5. Please kindly advise the source of Standard. synthesis ? or Recombinant ? or Extraction ? or other ?
6. If it is recombinant, please kindly advise the host.
7. Please kindly let us know whether the antibody and the standard includes any preservative or not.
ex. thimerosal or sodium azide or 2-mercaptoethanol etc.

A:
Thanks for your inquiry.
CSB-E13122h
Capture antibody is mouse monoclonal antibody and it is Protein A/G purified.
Detection antibody is goat polyclonal antibody and it is affinity purified..
Standard is 293 cell-derived recombinant protein.
This kit doesn't contain vaculovirus or other virus expressions；
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能：
    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 7512

    OMIM: 158370

    STRING: 9606.ENSP00000415183

    UniGene: Hs.315