Human Free Thyroxine,FT4 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

人游離甲狀腺素(FT4)酶聯(lián)免疫試劑盒（CSB-E05078h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的FT4含量。FT4即游離甲狀腺素，是甲狀腺功能檢查的重要指標(biāo)。它能反映甲狀腺的功能狀態(tài)，不受甲狀腺結(jié)合蛋白影響。其研究機(jī)制主要圍繞甲狀腺激素的合成、分泌、調(diào)節(jié)等過程，對診斷甲亢、甲減等甲狀腺疾病具有關(guān)鍵意義。試劑盒檢測范圍為4 pmol/L-64 pmol/L，該產(chǎn)品可應(yīng)用于科研領(lǐng)域中對甲狀腺功能調(diào)控機(jī)制的研究，例如甲狀腺功能亢進(jìn)/減退動(dòng)物模型評估、環(huán)境因素對甲狀腺激素代謝的影響分析，或藥物干預(yù)實(shí)驗(yàn)中激素水平變化的動(dòng)態(tài)監(jiān)測，為內(nèi)分泌系統(tǒng)相關(guān)基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

FT4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

4 pmol/L-64 pmol/L
靈敏度：

1.08 pmol/L
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-99
1:2	Average %	90
	Range %	87-100
1:4	Average %	95
	Range %	88-98
1:8	Average %	95
	Range %	86-98

回收率：

The recovery of human FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-98
EDTA plasma (n=4)	96	93-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.