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  4. Human Cystatin-F(CST7)ELISA kit

Human Cystatin-F(CST7)ELISA kit

  • 中文名稱：
    人胱抑素F(CST7)酶聯免疫試劑盒
  • 貨號：
    CSB-E17504h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人胱抑素F(CST7)酶聯免疫試劑盒（CSB-E17504h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液樣本中的CST7含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研場景中CST7的功能研究，例如探究其在炎癥反應、腫瘤侵襲或神經病變模型中的表達變化規律，或通過體外細胞培養實驗分析特定刺激下CST7的分泌動態，為疾病機制研究及潛在生物標志物探索提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    CMAP ELISA Kit; CST 7 ELISA Kit; Cst7 ELISA Kit; Cystatin 7 ELISA Kit; cystatin F (leukocystatin) ELISA Kit; Cystatin like metastasis associated protein ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-F ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; Cystatin7 ELISA Kit; CystatinF ELISA Kit; CYTF_HUMAN ELISA Kit; dJ568C11.1 ELISA Kit; Leukocystatin ELISA Kit
  • 縮寫：
    CST7
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 85-97
    1:2 Average % 98
    Range % 91-101
    1:4 Average % 94
    Range % 85-97
    1:8 Average % 90
    Range % 84-95
  • 回收率：
    The recovery of human CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 94-107
    EDTA plasma (n=4) 106 98-112
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.319 2.235 2.277 2.124
    20 1.685 1.671 1.678 1.525
    10 0.978 0.927 0.953 0.800
    5 0.641 0.627 0.634 0.481
    2.5 0.355 0.369 0.362 0.209
    1.25 0.247 0.260 0.254 0.101
    0.625 0.195 0.188 0.192 0.039
    0 0.151 0.155 0.153 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

Does your kit # CSB-E17504h or any of its components contain recombinant proteins?
If so, what is the expression system of these recombinants?

A:
Thanks for your inquiry!
CSB-E17504h contains recombinant proteins.
Standard is 293 cell-derived recombinant protein.

靶點詳情

  • 功能：
    Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
  • 基因功能參考文獻：
    1. during monocyte to macrophage differentiation, the endosomal/lysosomal proteolytic activity can be regulated by cystatin F whose expression is under the control of transcriptional factor C/EBP alpha. PMID: 30033148
    2. Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells. PMID: 22365146
    3. Transgenic cystatin F is expressed in model mice only during demyelination; in chronic demyelination, remyelinating ability is lost. PMID: 21344476
    4. cystatin F is an intracellular cysteine peptidase inhibitor with readily regulated expression PMID: 12423348
    5. CST7 is secreted, but artificial modification of its C-terminus can induce its endocytic targeting. PMID: 15212960
    6. Cystatin F was not colocalized with cystatin C shich suggests distinct functions for these two cysteine protease inhibitors in U937 cells. PMID: 15752368
    7. analysis of reduction-dependent activation of human cystatin F PMID: 16601115
    8. A latent protease inhibitor which is itself regulated by proteolysis in the endocytic pathway. PMID: 18256700
    9. Data demonstrate the addition of N-linked sugars to an Asn-X-Cys motif in cystatin F and suggest that the mannose 6-phosphate sorting machinery is used to divert cystatin F from the secretory pathway and to mediate its uptake from extracellular pools. PMID: 19192250

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  • 亞細胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    Cystatin family
  • 組織特異性：
    Primarily expressed in peripheral blood cells and spleen.
  • 數據庫鏈接：

    HGNC: 2479

    OMIM: 603253

    KEGG: hsa:8530

    STRING: 9606.ENSP00000420384

    UniGene: Hs.143212