Horse Progesterone(PROG) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

馬孕激素/孕酮(PROG)酶聯(lián)免疫試劑盒（CSB-E13183Hs）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的PROG含量。試劑盒檢測(cè)范圍為0.25 ng/mL-100 ng/mL，靈敏度為0.25 ng/mL。本試劑盒采用高特異性抗體，可精準(zhǔn)識(shí)別樣本中的孕酮分子，適用于科研場(chǎng)景下的馬科動(dòng)物生殖生理研究、妊娠狀態(tài)追蹤、繁殖效率評(píng)估及激素代謝分析。實(shí)驗(yàn)流程經(jīng)優(yōu)化設(shè)計(jì)，配套標(biāo)準(zhǔn)品及反應(yīng)體系完備，可在常規(guī)實(shí)驗(yàn)室條件下快速完成檢測(cè)，為畜牧業(yè)繁殖優(yōu)化、野生動(dòng)物保護(hù)等領(lǐng)域的激素水平研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

PROG
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma
檢測(cè)范圍：

0.25 ng/mL-100 ng/mL
靈敏度：

0.25 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-107
1:2	Average %	89
	Range %	83-99
1:4	Average %	95
	Range %	85-103
1:8	Average %	90
	Range %	86-102

回收率：

The recovery of horse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-106
EDTA plasma (n=4)	95	82-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average
100	0.162	0.158	0.160
18.75	0.320	0.332	0.326
3.125	0.542	0.563	0.553
0.875	0.971	0.944	0.958
0.25	1.503	1.485	1.494
0	2.192	2.127	2.160

Evaluation of circulating miRNAs in mares approaching parturition M Kikuchi, H Murase, K Urata, T Ishige, T Tozaki,Domestic Animal Endocrinology,2024

最新研究進(jìn)展：

PROG是一種激素，可通過(guò)作用于孕酮受體來(lái)調(diào)節(jié)生殖系統(tǒng)、乳房和神經(jīng)系統(tǒng)的功能。近年來(lái)，PROG被廣泛研究，尤其在抗腫瘤治療和神經(jīng)保護(hù)方面的應(yīng)用潛力備受關(guān)注。多項(xiàng)研究表明，PROG可以通過(guò)多種機(jī)制發(fā)揮抗腫瘤作用，如調(diào)節(jié)細(xì)胞周期、促進(jìn)細(xì)胞凋亡、抑制血管生成等。同時(shí)，PROG也具有保護(hù)神經(jīng)元、促進(jìn)神經(jīng)元再生的作用，可以減輕多種神經(jīng)退行性疾病的癥狀。這些研究為PROG的臨床應(yīng)用提供了新思路和方向。