Guinea pig tri-iodothyronine (T3) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

豚鼠三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒（CSB-EQ027510GU）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的T3含量。試劑盒檢測范圍為0.5 ng/mL-8 ng/mL，適用于科研場景中豚鼠甲狀腺功能研究、環(huán)境或藥物對內(nèi)分泌系統(tǒng)影響的評估，以及代謝性疾病模型的建立與機制探索，為動物實驗提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

T3
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

0.5 ng/mL-8 ng/mL
靈敏度：

0.25 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	95
	Range %	90-110
1:4	Average %	90
	Range %	83-99
1:8	Average %	97
	Range %	92-105

回收率：

The recovery of guinea pig T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-110
EDTA plasma (n=4)	94	89-102