Fish hepatocyte growth factor(HGF)ELISA Kit

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產品詳情

產品描述：

魚肝細胞生長因子(HGF)酶聯免疫試劑盒（CSB-E16527Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的HGF含量。HGF（肝細胞生長因子）是一種多功能細胞因子，主要來源于肝臟的Kupffer細胞、內皮細胞等，能刺激肝細胞增殖，并作用于上皮細胞、造血細胞、血管內皮細胞等多種細胞。HGF通過旁分泌或自分泌機制，在胚胎發生、創傷愈合、血管發生、組織器官再生等方面發揮重要作用。試劑盒檢測范圍為200 pg/mL-8000 pg/mL，適用于基礎醫學研究領域，例如肝臟疾病模型中的HGF動態監測、藥物干預效果評價或腫瘤微環境相關研究，也可用于細胞培養上清液中HGF分泌水平的定量分析，為探索HGF在病理生理過程中的調控機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

HGF
Uniprot No.：

F1REK1
種屬：

Fish
樣本類型：

serum, plasma
檢測范圍：

230 pg/mL-8000 pg/mL
靈敏度：

145 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-105
1:2	Average %	89
	Range %	85-100
1:4	Average %	89
	Range %	82-100
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of fish HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	95	89-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.