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  4. Duck Interferon γ,IFN-γ ELISA Kit

Duck Interferon γ,IFN-γ ELISA Kit

  • 中文名稱：
    鴨γ干擾素(IFN-γ)酶聯免疫試劑盒
  • 貨號：
    CSB-E08902Du
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    鴨γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E08902Du）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFN-含量。IFN-（干擾素相關靶點）在免疫調節中意義重大。其背景在于它與機體抗病毒、抗腫瘤等免疫反應密切相關。研究機制方面，主要是通過與細胞表面受體結合，激活相關信號通路，誘導多種抗病毒、免疫調節蛋白的表達，以發揮生物學效應。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研場景中鴨類病毒感染模型研究、疫苗免疫效果評價、免疫調節分子通路探索等方向本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫：
    IFN-
  • Uniprot No.：
  • 種屬：
    Duck
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of duck IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-98  
    1:2 Average % 98  
    Range % 92-106  
    1:4 Average % 85  
    Range % 81-86  
    1:8 Average % 88  
    Range % 83-94  
  • 回收率：
    The recovery of duck IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-94  
    EDTA plasma (n=4) 103 101-107  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.235 2.199 2.217 2.072  
    500 1.528 1.655 1.592 1.447  
    250 1.187 1.120 1.154 1.009  
    125 0.734 0.786 0.760 0.615  
    62.5 0.555 0.550 0.553 0.408  
    31.2 0.346 0.324 0.335 0.190  
    15.6 0.204 0.208 0.206 0.061  
    0 0.140 0.149 0.145    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 數據庫鏈接：