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  4. Dog Osteocalcin/bone gla protein,OT/BGP ELISA Kit

Dog Osteocalcin/bone gla protein,OT/BGP ELISA Kit

  • 中文名稱：
    犬骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13345c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯(lián)免疫試劑盒（CSB-E13345c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BGLAP含量。BGLAP即骨鈣素，是骨組織中最為豐富的非膠原蛋白。其背景上，與骨骼生長、代謝密切相關(guān)。研究機(jī)制方面，它可調(diào)節(jié)骨代謝平衡，還在能量代謝、生殖等過程中發(fā)揮作用，目前圍繞它在疾病診治中的潛在價(jià)值是研究熱點(diǎn)。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，科研人員可將其應(yīng)用于犬類骨骼發(fā)育研究、骨質(zhì)疏松模型構(gòu)建、骨代謝相關(guān)藥物篩選，以及營養(yǎng)干預(yù)對骨組織影響等基礎(chǔ)研究領(lǐng)域，為犬科動(dòng)物骨生物學(xué)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %87-102
    1:2Average %92
    Range %89-98
    1:4Average %89
    Range %84-96
    1:8Average %90
    Range %86-99
  • 回收率：
    The recovery of dog OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-96
    EDTA plasma (n=4)10295-109
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    203.105 3.004 3.055 2.953
    102.654 2.674 2.664 2.562
    51.878 1.889 1.884 1.782
    2.51.036 1.105 1.071 0.969
    1.250.468 0.500 0.484 0.382
    0.6250.261 0.273 0.267 0.165
    0.3120.158 0.164 0.161 0.059
    00.103 0.100 0.102 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 數(shù)據(jù)庫鏈接：