Canine trypsin ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒（CSB-E12804c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的trypsin含量。Trypsin即胰蛋白酶，是一種絲氨酸蛋白酶。它在人體消化過程中起關(guān)鍵作用，能水解蛋白質(zhì)。在研究方面，胰蛋白酶可作為藥物靶點，用于開發(fā)治療胰腺炎等疾病的藥物，同時也用于一些生物工藝，如細(xì)胞培養(yǎng)實驗中解離細(xì)胞。試劑盒檢測范圍為39 ng/mL-2500 ng/mL，該產(chǎn)品適用于科研領(lǐng)域中對犬類胰腺生理機(jī)制、炎癥反應(yīng)或營養(yǎng)代謝相關(guān)課題的探索，例如通過檢測不同實驗條件下胰蛋白酶表達(dá)動態(tài)，解析其與疾病模型的關(guān)聯(lián)性或評估藥物干預(yù)效果。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

trypsin
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

39 ng/mL-2500 ng/mL
靈敏度：

9.75 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	85-106
1:2	Average %	93
	Range %	85-105
1:4	Average %	95
	Range %	82-102
1:8	Average %	95
	Range %	84-110

回收率：

The recovery of canine trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-107
EDTA plasma (n=4)	97	86-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.