Canine inhibin beta-B (Inhbb)ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬抑制素β-B(Inhbb)酶聯(lián)免疫試劑盒（CSB-E15817c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Inhbb含量。犬抑制素β-B（Inhbb）是一種在多種惡性腫瘤中發(fā)揮重要作用的蛋白，其上調(diào)與胃癌患者預(yù)后不良相關(guān)。研究發(fā)現(xiàn)，Inhbb通過調(diào)節(jié)成纖維細(xì)胞的功能，影響腫瘤微環(huán)境，進(jìn)而促進(jìn)胃癌細(xì)胞增殖、遷移和侵襲。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，該產(chǎn)品廣泛應(yīng)用于生殖生物學(xué)、內(nèi)分泌學(xué)研究領(lǐng)域，特別適用于卵巢功能評估、睪丸發(fā)育調(diào)控、妊娠相關(guān)激素變化等科研方向，亦可作為生殖系統(tǒng)疾病動物模型的生物標(biāo)志物檢測工具，為犬類繁殖力研究及生殖健康機(jī)制探索提供可靠的技術(shù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Inhbb
Uniprot No.：

F1PB74
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

5 pg/mL-1000 pg/mL
靈敏度：

2.5 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine Inhbb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-104
1:2	Average %	90
	Range %	85-94
1:4	Average %	92
	Range %	87-96
1:8	Average %	100
	Range %	96-104

回收率：

The recovery of canine Inhbb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	92-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.