Canine Progesterone,PROG ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬孕激素/孕酮(PROG)酶聯免疫試劑盒（CSB-E07285c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的PROG含量。試劑盒檢測范圍為0.15 ng/ml-70 ng/ml，靈敏度為0.2 ng/ml。適用于動物繁殖生理研究、生殖內分泌機制探索、藥物對卵巢功能影響評估等場景，尤其可為犬類人工授精時機判斷、妊娠監測及生殖疾病模型構建提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PROG
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates.
檢測范圍：

0.15 ng/ml-70 ng/ml.
靈敏度：

0.2 ng/ml.
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	94
	Range %	91-99
1:4	Average %	102
	Range %	98-105
1:8	Average %	89
	Range %	82-94

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

2.284

2.275

2.280

0.15

1.429

1.589

1.509

0.843

0.874

0.859

0.566

0.585

0.576

0.362

0.353

0.358

0.201

0.202