Canine Creatine Kinase MB isoenzyme (CK-MB)ELISA Kit

說明書下載

產品詳情

產品描述：

犬肌酸激酶同工酶MB(CK-MB)酶聯免疫試劑盒（CSB-E15852c）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶MB，主要存在于心肌中。在急性心肌梗死等心肌損傷時，其釋放入血，水平會顯著升高。研究機制方面，通過檢測血液中CK-MB活性或含量變化，可輔助診斷心肌損傷情況、判斷病情發展及評估治療效果。試劑盒檢測范圍為1.56 μIU/mL-100 μIU/mL，適用于犬類模型的心肌損傷相關科研實驗，例如評估心肌缺血再灌注損傷程度、監測心臟毒性藥物對心肌的影響或探究心臟疾病模型的病理進程。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CK-MB
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

1.56 μIU/mL-100 μIU/mL
靈敏度：

0.78 μIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	98
	Range %	94-102
1:400	Average %	90
	Range %	86-94
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of canine CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-107
EDTA plasma (n=4)	89	85-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.