Bovine Transthyretin(TTR) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛運甲狀腺素蛋白(TTR)酶聯(lián)免疫試劑盒（CSB-EL025270BO）為競爭法 ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TTR含量。TTR（轉(zhuǎn)甲狀腺素蛋白）是一種血漿運載蛋白。其基因突變可致蛋白構(gòu)象改變、聚集和沉積，引發(fā)ATTR淀粉樣變性病，危害神經(jīng)系統(tǒng)、心臟等。目前研究聚焦于穩(wěn)定TTR結(jié)構(gòu)、抑制聚集等機制，以開發(fā)針對性治療藥物。試劑盒檢測范圍為312.5 ng/mL-5000 ng/mL，該產(chǎn)品適用于牛類生理機制研究、疾病模型建立或營養(yǎng)代謝實驗中TTR蛋白動態(tài)變化的追蹤，為動物科學(xué)、獸醫(yī)學(xué)及比較醫(yī)學(xué)領(lǐng)域的科研人員提供可靠的檢測工具，助力探索TTR在跨膜運輸、能量代謝或組織特異性表達中的生物學(xué)功能。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TTR ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit
縮寫：

TTR
Uniprot No.：

O46375
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

312.5 ng/mL-5000 ng/mL
靈敏度：

312.5 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-97
1:200	Average %	103
	Range %	99-107
1:400	Average %	98
	Range %	93-103
1:800	Average %	92
	Range %	87-95

回收率：

The recovery of bovine TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	89	84-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
亞細胞定位：

Secreted.
蛋白家族：

Transthyretin family
組織特異性：

Detected in serum (at protein level).
數(shù)據(jù)庫鏈接：

KEGG: bta:280948

STRING: 9913.ENSBTAP00000014585

UniGene: Bt.207