Bovine Beta-Endorphin,β-EP ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒（CSB-E13051B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的β-EP含量。β - EP即β - 內(nèi)啡肽，是人體自身產(chǎn)生的具有強大鎮(zhèn)痛作用的內(nèi)源性活性物質(zhì)。其研究機制主要圍繞它與體內(nèi)阿片受體結(jié)合，調(diào)節(jié)痛覺傳導(dǎo)、情緒等生理過程，在鎮(zhèn)痛、精神疾病等領(lǐng)域研究較多，對理解人體生理和開發(fā)相關(guān)療法有重要意義。試劑盒檢測范圍為46.88 pg/mL-3000 pg/mL，可為神經(jīng)生物學(xué)、疼痛機制研究或應(yīng)激反應(yīng)動物模型提供數(shù)據(jù)支持，例如探究藥物干預(yù)、環(huán)境刺激對β - EP表達(dá)水平的影響；適用于牛源樣本的體外定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

beta-Endorphin, beta-EP
縮寫：

β-EP
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

46.88 pg/mL-3000 pg/mL
靈敏度：

11.72 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	84
	Range %	80-88
1:4	Average %	92
	Range %	88-97
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.