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  4. CYC1 Recombinant Monoclonal Antibody

CYC1 Recombinant Monoclonal Antibody

  • 中文名稱：
    CYC1 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA006327MA1HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate(20μg), PC-3 whole cell lysate(20μg), HeLa whole cell lysate(20μg), K562 whole cell lysate(20μg), HepG2 whole cell lysate(20μg), Jurkat whole cell lysate(20μg), SH-SY5Y whole cell lysate(20μg)
      All lanes: CYC1 antibody at 1:1000
      Secondary
      Goat polyclonal to human IgG at 1/40000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa
      Exposure time：1min
    • Overlay Peak curve showing PC-3 cells stained with CSB-RA006327MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Complex III subunit 4 antibody; Complex III subunit IV antibody; CY1_HUMAN antibody; CYC1 antibody; Cytochrome b c1 complex subunit 4 antibody; Cytochrome b-c1 complex subunit 4 antibody; Cytochrome bc1 complex subunit 4 antibody; Cytochrome c 1 antibody; Cytochrome c-1 antibody; Cytochrome c1; heme protein; mitochondrial antibody; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit antibody; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit antibody; UQCR4 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human CYC1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    hIgG1
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    27E12D11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.
  • 基因功能參考文獻(xiàn)：
    1. these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
    2. results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
    3. CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
    4. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
    5. our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
    6. Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
    7. HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
    8. Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
    9. Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
    10. the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
    11. Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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  • 相關(guān)疾病：
    Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)
  • 亞細(xì)胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome c family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2579

    OMIM: 123980

    KEGG: hsa:1537

    STRING: 9606.ENSP00000317159

    UniGene: Hs.289271