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  4. Sheep Interleukin 1β,IL-1β ELISA Kit

Sheep Interleukin 1β,IL-1β ELISA Kit

  • 中文名稱：
    綿羊白介素1β(IL-1β)酶聯免疫試劑盒
  • 貨號：
    CSB-E10115Sh
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    綿羊白介素1β(IL-1β)酶聯免疫試劑盒（CSB-E10115Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B，即白細胞介素-1β，是一種重要的促炎細胞因子，參與先天免疫反應。它由免疫和非免疫細胞在炎癥信號刺激下產生，通過炎癥小體激活和蛋白水解加工，與多種細胞因子協同作用，調節炎癥和免疫反應，對腫瘤的發生、發展和轉移具有重要作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，科研人員可借助定量分析感染模型、自身免疫疾病研究或藥物干預實驗中不同生物樣本的IL - 1β濃度變化，為炎癥機制探索、免疫調控研究及生物制劑開發提供可靠數據支持。所有檢測結果僅用于科學研究目的。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Ovis aries (Sheep)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %83-90
    1:2Average %98
    Range %92-102
    1:4Average %100
    Range %96-104
    1:8Average %94
    Range %90-96
  • 回收率：
    The recovery of sheep IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9794-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.487 2.456 2.472 2.371
    5002.031 2.000 2.016 1.915
    2501.614 1.568 1.591 1.490
    1251.105 1.023 1.064 0.963
    62.50.703 0.689 0.696 0.595
    31.20.421 0.402 0.412 0.311
    15.60.275 0.262 0.269 0.168
    00.103 0.099 0.101 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能參考文獻：
    1. Intravenous injection of SB203580 successfully inhibited synthesis of IL-1beta and reduced the production of IL-6 in the hypothalamus. PMID: 24995301
    2. Interleukin-1beta was increased only on the injured side of multifidus in disc disease. PMID: 24718080
    3. Inactivation of RhoA due to lack of prenylation leads to Rac1 activation and subsequent priming for IL-1beta secretion. PMID: 25107911
    4. RANTES-403 was significantly associated with the risk of developing diffuse-type gastric cancer in men and found a possible gene-gene interaction between RANTES and IL-1B polymorphisms in gastric cancer carcinogenesis. PMID: 24323564
    5. In vitro studies with IL-1-beta and TNF-alpha challenges, both proinflammatory cytokines resulted in elevated levels of CCL5 in conditioned media (p<0.01) PMID: 24468005
    6. These results showed that IL-1beta and IL-1 receptor types I and II were expressed in the ovine hypothalamus in response to lipopolysaccharide injection. PMID: 20345592
    7. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
    8. plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID: 17221363

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  • 亞細胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數據庫鏈接：

    KEGG: oas:443539

    UniGene: Oar.434