Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

小鼠白介素1β前體(pro-IL1B)酶聯(lián)免疫試劑盒（CSB-ET011614MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的pro-IL1B含量。pro-IL1B，即前白細(xì)胞介素-1β，是一種無(wú)活性的前體蛋白，由pro-Il-1b基因編碼。它在炎癥刺激下被激活，裂解為具有生物活性的IL-1β，參與炎癥和免疫反應(yīng)。研究顯示，pro-IL1B在腫瘤發(fā)生發(fā)展中扮演重要角色，通過(guò)促進(jìn)炎癥反應(yīng)和腫瘤血管生成，影響腫瘤的侵襲和轉(zhuǎn)移。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于體外炎癥模型構(gòu)建、免疫調(diào)控機(jī)制研究、藥物抗炎效果評(píng)估等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

pro-IL1B
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-113
1:2	Average %	87
	Range %	82-91
1:4	Average %	94
	Range %	90-99
1:8	Average %	85
	Range %	80-90

回收率：

The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	91	85-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.