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  4. Canine calprotectin (CALP) ELISA kit

Canine calprotectin (CALP) ELISA kit

  • 中文名稱：
    犬鈣衛蛋白(CALP)酶聯免疫試劑盒
  • 貨號：
    CSB-EQ013485DO
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    犬鈣衛蛋白(CALP)酶聯免疫試劑盒（CSB-EQ013485DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CALP含量。犬鈣衛蛋白（CALP）是一種在犬類體內發揮重要生理功能的蛋白質，參與細胞分化、免疫調節、腫瘤發生等過程。其研究機制涉及炎癥反應和疾病診斷，如通過ELISA試劑盒檢測犬血清和唾液中的CALP濃度，以評估炎癥水平和疾病狀態。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，采用預包被高特異性抗體，操作簡便，兼容多樣本類型，可滿足實驗室對犬類炎癥相關疾病的機制研究、藥物干預效果評估或動物模型分析等科研需求。通過標準化的檢測流程，研究者可高效獲取重復性良好的數據，適用于體外實驗中探索CALP在犬免疫應答、組織損傷修復等領域的生物學功能。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    CALP
  • 種屬：
    Canine
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 86    
    Range % 81-92    
    1:2 Average % 102    
    Range % 97-107    
    1:4 Average % 96    
    Range % 85-99    
    1:8 Average % 103    
    Range % 96-107    
  • 回收率：
    The recovery of canine CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 94 89-98    
    EDTA plasma (n=4) 102 97-106    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    400 1.816 1.921 1.869 1.723    
    200 1.448 1.512 1.480 1.334    
    100 1.030 1.078 1.054 0.908    
    50 0.758 0.733 0.746 0.600    
    25 0.476 0.466 0.471 0.325    
    12.5 0.348 0.332 0.340 0.194    
    6.25 0.275 0.268 0.272 0.126    
    0 0.145 0.146 0.146 　    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

Would you provide the information:
1) antibody clonality _ are capture and detection antibodies monoclonal or polyclonal?
2) antibody's target immunogen _ to which immunogen have detection and capture antibodies of the kit been raised? was it of human or canine origin? was it a native or recombinant protein? was it a full length protein or a fragment?
3) proteins detected in the samples by the antibodies _ do the
antibodies of the kit detect only the S100A8/S100A9 heterocomplex or do they also detect S100A8 and S100A9 proteins separately (as monomers or homodimers)?
4) standard protein _ is the protein used as the analytical standard of the kit of canine or human origin? is it a native or recombinant protein? is it a full length protein or a fragment?

A:
Thanks for your inquiry!CSB-EQ013485DO detects CALP(Calprotectin is a complex of the mammalian proteins S100A8 and S100A9)
The kit uses 2 antibodies, the Coated anti. and Detect anti. are both Goat polyclonal antibody, which are designed for the information of the links
https://www.ncbi.nlm.nih.gov/protein/XP_013970901.1 and https://www.ncbi.nlm.nih.gov/protein/NP_001139616.1 separately.
The immunogen is 293 cell-derived recombinant protein.
The standard is the purified crude product of the natural sample,but so sorry the specific source information can not be disclosed.