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  4. Bovine Interleukin 1β (IL- 1β) ELISA kit

Bovine Interleukin 1β (IL- 1β) ELISA kit

  • 中文名稱：
    牛白介素1β(IL-1β)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12986B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E12986B）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL1B含量。IL-1β（IL1B）是一種重要的促炎細(xì)胞因子，在炎癥反應(yīng)、免疫調(diào)節(jié)等生理和病理過程中起關(guān)鍵作用。研究機(jī)制方面，它能激活下游信號(hào)通路，引發(fā)多種免疫細(xì)胞的活化，與多種炎性疾病如類風(fēng)濕關(guān)節(jié)炎等的發(fā)病機(jī)制密切相關(guān)，是熱門治療靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，科研人員可將其應(yīng)用于牛源性疾病模型構(gòu)建、疫苗免疫效果評(píng)估、炎癥相關(guān)信號(hào)通路研究等方向，例如通過檢測(cè)感染或應(yīng)激條件下生物樣本中IL - 1β的動(dòng)態(tài)變化，揭示免疫調(diào)控機(jī)制或篩選抗炎藥物作用靶點(diǎn)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL1B ELISA Kit; Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %99
    Range %95-108
    1:2Average %94
    Range %91-98
    1:4Average %92
    Range %88-95
    1:8Average %87
    Range %81-93
  • 回收率：
    The recovery of bovine IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-102
    EDTA plasma (n=4)9287-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.876 2.862 2.869 2.751
    20002.444 2.439 2.442 2.324
    10001.903 1.856 1.880 1.762
    5001.412 1.403 1.408 1.290
    2501.164 1.185 1.175 1.057
    1250.809 0.799 0.804 0.686
    62.50.471 0.479 0.475 0.357
    00.117 0.118 0.118 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能參考文獻(xiàn)：
    1. Mycobacterium bovis infected animals have a higher frequency of IFN-gamma producing CD4(+) T cells and elevated plasma IL-1beta levels. PMID: 28610788
    2. The presence of embryos increased endometrial IL1B protein locally, while no differences regarding IL1R1 protein and IL1B and IL1R1 mRNA were detected. PMID: 25955718
    3. For the inflammasome-dependent IL-1beta release, bovine monocytes require ATP in addition to a primary stimulus. This IL-1beta release depends on potassium efflux, but, in contrast to human and murine monocytes, does not require calcium influx or generation of reaction oxygen and is independent of the P2X7 receptor. PMID: 22728096
    4. Role of TGF-beta1 and TNF-alpha in IL-1beta mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease. PMID: 21722622
    5. Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
    6. Data describe the expression of IL-8, IL-1beta and their respective receptors, CXCR1 and IL-1R1 in bovine theca cells, and suggest that VEGF is associated with the IL system in theca cells in ovary. PMID: 20156197
    7. Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase. PMID: 15253926
    8. non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties PMID: 17174540
    9. Lipopolysaccharide stimulation up-regulates the secretion of cytokines by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
    10. dynamic compression stimulates cell proliferation and proteoglycan synthesis in the presence of IL-1beta and/or inhibitors of the MAPKs and NFkappaB and AP-1 signalling pathways PMID: 18545934
    11. These results indicate that activation of the intrinsic antistaphylococcal response in bovine endothelial cells (BEC), enhanced by TNF-alpha and IL-1beta, is effective to eliminate S. aureus and S. epidermidis. PMID: 18625014
    12. the low friction of articular cartilage can be modified by TGF-beta1 and IL-1beta treatment and that the friction coefficient depends on multiple factors, including superficial zone protein localization and surface roughness PMID: 18683879
    13. These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells. PMID: 18930361
    14. Data suggest an important regulatory mechanism of interleukin-1beta production, associated with microglial migration and proliferation, driven by ECM degradation and/or synthesis in an injured brain. PMID: 19250967

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  • 亞細(xì)胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:281251

    STRING: 9913.ENSBTAP00000001745

    UniGene: Bt.4856